Rapid characterization of microorganisms by mass spectrometry

by Catherine Fenselau

Publisher: American Chemical Society in Washington, DC

Written in English
Cover of: Rapid characterization of microorganisms by mass spectrometry | Catherine Fenselau
Published: Downloads: 373
Share This

Subjects:

  • Identification,
  • Microbiological chemistry,
  • Microorganisms,
  • Mass spectrometry

Edition Notes

StatementCatherine Fenselau, editor, Plamen Demirev, editor
SeriesACS symposium series -- 1065
ContributionsAmerican Chemical Society. Division of Analytical Chemistry
Classifications
LC ClassificationsQR69.M33 R37 2011
The Physical Object
Paginationp. cm.
ID Numbers
Open LibraryOL25077782M
ISBN 109780841226128
LC Control Number2011017703

The application of mass spectrometry (MS) in the identification and chemical characterization of microorganisms is discussed. An overview of MS in microbial characterization is given followed by 13 chapters which discussed the detection of microorganisms in clinical and environmental matrices, and the use of MS in identifying key chemical components of microoorganisms with reference to.   Read "Book Review: Identification of Microorganism by Mass Spectrometry – Chemical Analysis: A series of Monographs on Analytical Chemistry and its Applications (Series Editor: J. D. Winefordner). By C. L. Wilkins and J. O. Lay, jr. (Eds.)., Engineering in Life Sciences" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available . Applications of mass spectrometry in microbiology: from strain characterization to rapid screening for antibiotic resistance. Responsibility Plamen Demirev, Todd R. Sandrin, editors. Developments of novel MS-based assays for rapid identification of strains of antibiotic-resistant microorganisms are reviewed in the book as well. Mass spectrometry (MS) is widely used for characterization of microorganisms ().Success in characterization of microorganisms and in differentiation between species and strains hinges upon the detection of an ample number of unique biomarkers in the desired m/z range. Various sources of such biomarkers have been exploited, including cell wall lipids (15, 17), carbohydrates (), nucleic acids.

Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS), in combination with Biotyper software, is a rapid, high-throughput, and accurate method for the identification of microbes. Microbial outbreaks in a brewery present a major risk for companies as it can lead to cost-i Lab on a Chip Recent HOT Articles. Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria J Microbiol Methods. Mar;92(3) doi: /   (1.) Demirev PA, Fenselau C. Mass spectrometry for rapid characterization of microorganisms. Annu Rev Anal Chem ; (2.) Gu H, Ho P-L, Tsang KWT, Wang L, Xu B. Using biofunctional magnetic nanoparticles to capture vancomycin-resistant Enterococci and other gram-positive bacteria at ultralow concentration. J Am Chem Soc ; applications of mass spectrometry in microbiology from strain characterization to rapid screening for antibiotic resistance Posted By Anne Golon Publishing TEXT ID caa7c3 Online PDF Ebook Epub Library revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy rapid high throughput low cost and efficient.

Microorganism Identification by Mass Spectrometry and Protein Database Searches Plamen A. Demirev,* Yen-Peng Ho, Victor Ryzhov, and Catherine Fenselau Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland A method for rapid identification of microorganisms is.   Astrobiology Vol. 9, No. 8 Rapid Communications. Characterization of Organics, Microorganisms, Desert Soils, and Mars-like Soils by Thermal Volatilization Coupled to Mass Spectrometry and Their Implications for the Search for Organics . Rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl. Environ. Microbiol. –

Rapid characterization of microorganisms by mass spectrometry by Catherine Fenselau Download PDF EPUB FB2

: Rapid Characterization of Microorganisms by Mass Spectrometry (ACS Symposium Series) (): Fenselau, Catherine, Demirev, Plamen: Books. This book contains chapters from participants in a recent symposium, organized by the Division of Analytical Chemistry during the ACS National Meeting in Washington DC in August,as well as several other active researchers in the field.

The book covers aspects of mass spectrometry (MS) applications for microorganism characterization in several fields: biodefense, clinical diagnostics. Strategies for the rapid and reliable analysis of microorganisms have been sought to meet national needs in defense, homeland security, space exploration, food and water safety, and clinical diagnosis.

Mass spectrometry has long been a candidate technique because it is extremely rapid and can provide highly specific information.

It has excellent sensitivity. Molecular and fragment ion masses Cited by: Rapid characterization of microorganisms by mass spectrometry: an overview / Catherine Fenselau and Plamen Demirev --Rapid sample preparation for microorganism analysis by mass spectrometry / Franco Basile --Discrimination of fungi by MALDI-TOF mass spectrometry / Justin M.

Hettick [and others] --Fungal metabolites for microorganism. The book covers aspects of mass spectrometry (MS) applications for microorganism characterization in several fields: biodefense, clinical diagnostics, food safety, environmental monitoring, and chemotaxonomy/biosystematics.

MALDI mass spectrometry-based systems for rapid characterization of microorganisms in biodefense or medical diagnostics usually detect intact proteins in theDa : Catherine Fenselau.

Although strategies exist by which mass spectrometry can be used for rapid characterization of small RNA and DNA fragments, protein analysis is the significant product of three decades of development of mass spectrometry for rapid analysis of microorganisms.

The comparison of MS-based protein analysis with PCR is not straight-forward. In this chapter, the use mass of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is discussed as an emerging technology for the rapid characterization.

About this book A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and.

Xanthii Fructus (XF) is known as a medicinal plant. It has been used as a traditional medicine because of its high biological efficacy. However, there have been few comprehensive studies on the specific chemical composition of the plant and consequently, the information is lacking for the mechanism of the natural product metabolites in humans.

Rapid characterization of microorganisms by mass spectrometry book In this study, an efficient analytical method to.

Such top-down or bottom-up proteomics approaches permit rapid, sensitive, and confident characterization of individual microorganisms in mixtures and are reviewed here. Examples of MS-based functional assays for detection of targeted microorganisms, e.g., Bacillus anthracis, in environmental or clinically relevant backgrounds are also by: Summary Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) for characterization and analysis of microorganisms, specifically bacteria, is described here as a rapid screening tool.

A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms.

Rapid Sample Preparation for Microorganism Analysis by Mass Spectrometry Franco Basile Chapter 2, DOI: /bkch Publication Date (Web): June 6,   In parallel, with the expansion of MS technologies in the “omics” fields, novel MS‐based research efforts to characterize organismal as well as environmental microbiomes have emerged.

Successful characterization of microorganisms found in complex mixtures of other organisms remains a major challenge for researchers and clinicians alike. Advances in instrumentation, proteomics, and bioinformatics have contributed to the successful applications of mass spectrometry (MS) for detection, identification, and classification of microorganisms.

These MS applications are based on the detection of organism-specific biomarker molecules, which allow differentiation between organisms to be made. Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance: Medicine & Health Science Books @ Strategies for the rapid and reliable analysis of microorganisms have been sought to meet national needs in defense, homeland security, space exploration, food and water safety, and clinical diagnosis.

Mass spectrometry has long been a candidate technique because it is extremely rapid and can provide highly specific information. It has excellent sensitivity. Rapid isolation and characterization of bacterial lipopeptides using liquid chromatography and mass spectrometry analysis The structural analysis of post-translational modifications (PTMs) of lipoproteins is difficult due to the hydrophobic properties of their fatty acid moieties.

Due to rapid progress in the development of mass spectrometry devices during the last few years, identification by means of mass spectrometry has become a very powerful and usable tool. These methods offer fast analysis of biomarker ions, providing reliable information on bacteria characterization even at the sub-species level.

Rapid screening and characterization of bacteria associated with hospital cockroaches (Blattella germanica L.) using MALDI‐TOF mass spectrometry A. Mehainaoui Department of Biochemistry, Faculty of Science, University Badji Mokhtar Annaba, Annaba, Algeria.

The emergence of new, antibiotic-resistant strains of human or animal pathogens is of extraordinary concern not only to the scientific and medical communities, but to the general public as well.

Developments of novel MS-based assays for rapid identification of strains of antibiotic-resistant microorganisms are reviewed in the book as well.

For the past three decades, a number of mass spectrometry-based methods have been developed for the rapid differentiation of bacteria phenotypically. Ionization of bacterial extracts by fast-atom bombardment, electrospray ionization, and sample pyrolysis, followed by MS and MS/MS, have been reported (2, 6, 20, 31).

Rapid evaporative-ionization mass spectrometry (REIMS) coupled with an electroknife as a sampling device was recently employed in many application fields to obtain a rapid characterization of different samples without any need for extraction or cleanup procedures.

In the present research, REIMS was used to obtain a metabolic profiling of the Kigelia africana fruit, thus extending the. applications of mass spectrometry in microbiology from strain characterization to rapid screening for antibiotic resistance Posted By J. Tolkien Media Publishing TEXT ID caa7c3 Online PDF Ebook Epub Library from strain characterization to rapid screening for antibiotic resistance hardcover new pp applications of mass spectrometry in microbiology from strain.

Abstract. A technique is described for detecting and characterizing bacteria on a single-particle basis by mass spectrometry. The method involves generation of a particle beam of single whole cells which are rapidly volatilized and ionized in vacuum in the ion source of a quadrupole mass spectrometer.

In the last quarter century, advances in mass spectrometry (MS) have been at the forefront of efforts to map complex biological systems including the human metabolome, proteome, and microbiome.

All of these developments have allowed MS to become a well-established molecular level technology for microorganism characterization. applications of mass spectrometry in microbiology from strain characterization to rapid screening for antibiotic resistance Posted By Horatio Alger, Jr.

Publishing TEXT ID caa7c3 Online PDF Ebook Epub Library measure the mass to charge m z ratios of the resulting ions with the combination of the mass spectrometer computer technology and the software the range of applications.

Find many great new & used options and get the best deals for Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance (, Hardcover) at the best online prices at eBay. Free shipping for many products. Identification of Microorganisms by Mass Spectrometry Method Reproducibility and Spectral Library Assembly for Rapid Bacterial Characterization by Metastable Atom Bombardment Pyrolysis Mass Spectrometry.

click here to see books videos cd-roms of related interest. A technique is described for detecting and characterizing bacteria on a single-particle basis by mass spectrometry.

The method involves generation of a particle beam of single whole cells which are rapidly volatilized and ionized in vacuum in the ion source of a quadrupole mass spectrometer.

MALDI-TOF-MS is a rapid, precise, and cost-effective method for identification of intact bacteria, compared to conventional phenotypic techniques or molecular biology. Furthermore, it allows identification of bacteria directly from clinical samples (blood cultures for example).One technology potentially capable of meeting these needs is a high-throughput mass spectrometry (MS)-based proteomic approach.

This approach utilizes the knowledge of amino acid sequences of peptides derived from the proteolysis of proteins as a basis for reliable bacterial identification.